It is not NLM practice, but an issue number may be omitted if the journal is continuously paginated throughout the volume. However, note that many libraries such as the NLM require issue numbers for retrieval from their collections and for interlibrary loan.
Nallamothu BK, Bradley EH, Krumholz HM. Time to treatment in primary percutaneous coronary intervention. N Engl J Med. 2007 Oct 18;357:1631-8.
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Citing Medicine: The NLM Style Guide for Authors, Editors, and Publishers [Internet]. 2nd edition. Chapter 1: Journals
Related:
General Rules for Issue Number (required) for Journal Articles Vancouver Style
Specific Rules #1 for Issue Number (required) for Journal Articles Vancouver Style: Non-English names for issue
Specific Rules #2 for Issue Number (required) for Journal Articles Vancouver Style: No volume number present
Specific Rules #3 for Issue Number (required) for Journal Articles Vancouver Style: No issue number present
Specific Rules #4 for Issue Number (required) for Journal Articles Vancouver Style: Options for issues
Specific Rules #5 for Issue Number (required) for Journal Articles Vancouver Style: Further subdivisions to supplements, parts, etc., to an issue
Examples for Issue Number (required) for Journal Articles Vancouver Style
General Rules for Supplement/Part/Special Number to an Issue (required) for Journal Articles Vancouver Style
Specific Rules for Supplement/Part/Special Number to an Issue (required) for Journal Articles Vancouver Style
Examples for Supplement/Part/Special Number to an Issue (required) for Journal Articles Vancouver Style
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